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The beads which might be negatively charged are called cation exchange resins, as positively charged proteins will likely be attracted. For example, in a sulphonated polystyrene resin, a cation exchange resin, the chlorine ion of a hydrochloric acid buffer ought to equilibrate into the resin. This effect comes because of two equally charged particles, one from the resin and one from the solution, failing to distribute properly between the two sides; there's a selective uptake of 1 ion over another.

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This behavior vapereason was manipulated to separate lipids, primarily fatty acids from mixtures in to fractions with differing variety of double bonds utilizing silver ions. Elution from ion-alternate columns will be delicate to changes of a single charge- chromatofocusing.

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